#3650 LINACLOTIDE IS PROTECTIVE AGAINST RENAL ISCHEMIA-REPERFUSION INJURY

Abstract Background and Aims Renal ischemia-reperfusion injury is associated with delayed graft function poor long-term survival following transplantation. Linaclotide a guanylate cyclase C (GC-C) receptor agonist that increases intracellular cGMP concentrations promotes intestinal transport capacity by activating the GC-C receptor. Since CKD deficiency of in kidney, increasing kidney linaclotide would suppress fibrosis, protecting kidney. Method An AKI rat model unilateral nephrectomy plus contralateral ischaemia (30 min) impaired reperfusion (up to 14 days) was used. administered before after surgery for 2 weeks, blood renal tissue samples were collected determine its effect on whether it protective against progression CKD. Results In I/R injury+ group, creatinine levels lower linaclotide-treated group than non-treated group. Sirius red staining showed significant improvement fibrosis. The hydroxyproline content kidneys determined basic method. RNA seq genes down-regulated oxidative stress markers SOD3 Gpx1. expression up-regulated degree similar injury- treatment linaclotide. These data suggest may ameliorate fibrosis suppressing dysfunction through antioxidant effects. Conclusion found decrease caused I/R. We like apply this finding human transplantation future prevent deterioration due